Agriculture is the foundation of human life, and the cultivation of genetic plants injects new vitality into agricultural production. Transgenic plants refer to the integration of artificially isolated or modified genes into the genome of target crops through genetic engineering methods to achieve increased crop yields, increased crop resistance, increased resistance to diseases and insect pests, and increased nutritional content. The cultivation of genetically modified crops can improve agricultural production efficiency, reduce the amount of herbicides and pesticides, increase crop yields, improve the ecological environment, improve the quality of human life, improve the nutritional structure of the human body, and assist in the prevention of certain diseases. Gene engineering technology in a broad sense includes transgenic technology and gene editing technology. Transgenic technology directly introduces the target gene to accurately locate and change the type of genetic traits.
With the introduction of gene editing technology, the breeding space of crops has been further expanded, the designability of crop genomes has been enhanced, and the breeding bottleneck has achieved a great breakthrough. Compared with traditional breeding methods, genetic engineering technology has higher purpose and selectivity, greatly reducing the time required to identify and isolate the genetic material that determines good quality, and at the same time, it can efficiently achieve good quality cross-species transfer and gene transfer. Self-designed and synthesized is an important supplement and development of traditional breeding methods. A revolutionary method of gene synthesis will allow faster development in almost every imaginable way.
The general breeding process of transgenic plants is as follows. In the process of transgenic plants breeding, the high-throughput htDNA-chip® technology platform can construct a customized library for target gene screening and isolation, high-throughput synthetic DNA, and use next-generation sequencing technology to identify whether the target gene has been successfully introduced.
Fig.1 Transgenic plants cultivation process
Based on our silicon-based htDNA-chip® technology platform, thousands of nucleotide chains can be synthesized in a single run. On the one hand, for target genes with known sequences, high-throughput amplification can be carried out directly through htDNA-chip®. On the other hand, for unknown genes, htDNA-chip® can conduct target genes screening by establishing a genome-wide library or customizing a library you need. High-throughput, high-fidelity gene fragments synthesis and the establishment of customizable libraries will greatly improve the breeding process of plants.
In the breeding process of transgenic plants, there is a certain degree of uncertainty due to the successful introduction of the target gene. Because the combination of the target gene and the recipient cell does not mean that the transgene is successful, there is a certain probability of transformation. At this time, the next-generation sequencing technology of htDNA-chip® is needed to confirm that the target gene has been successfully integrated into the recipient cell genome. In the early stage of seedling raising, such screening can greatly reduce unnecessary waste of resources. htDNA-chip® ensures that the entire process is high-throughput and can be screened when the sample volume is large enough. This technology platform uses next-generation sequencing to identify and verify genetically modified plants, which greatly promotes the breeding process.
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