DNA-encoded chemical library technology is increasingly being used for lead compound screening to accelerate the development of new drugs. It uses DNA fragments as barcodes to record the structural information of compounds. In a compound library containing hundreds of millions of chemical molecules, each molecule is linked to a unique piece of DNA. The DNA coding compound library finally realizes the coding of each compound in the compound library. As you can see in the below picture, in a DNA-encoded library, each compound is covalently attached to a unique DNA tag, serving as the identifier of the chemical structure of the compound. After selection against biological targets, the chemical structure of selected compounds can be decoded by reading the sequence of the DNA tag via PCR amplification and the ultra-high-throughput DNA sequencing technology.
Fig.1 Scheme for the selection of DNA-encoded chemical library against biological targets(Shi, B.; et al, 2017)
In the process of DNA-encoded compound library in new drug research, htDNA-chip® technology platform is mainly capable of high-throughput next-generation sequencing and rapid library customization owing to high-fidelity DNA coding design and synthesis. htDNA-chip® can promote the construction of DNA-encoded compound libraries, and further promote the discovery, screening and optimization of lead drugs.
The silicon-based high-throughput htDNA-chip® technology platform can meet the needs of a large number of special DNA sequence coding in the process of building a DNA-encoded compound library in a single run. Through htDNA-chip®, you can easily encode the huge compound library. In addition, the high flexibility of htDNA-chip® ensures that you can build a DNA-encoded compound library at any performance. So you can fully utilize your design inspiration and imagination. High-throughput and high-precision htDNA-chip® lays a solid foundation for your new drug discovery.
In the DNA-encoded compound library, each unique DNA sequence encodes a specific compound. There is a one-to-one correspondence between the structural units of the compound and the DNA sequence. After affinity screening with the corresponding target, the structural information of the compound can be identified through the recognition of the DNA sequence. Then the translated compound can be synthesized, and the activity confirmed with the target. Finally, the lead compound is obtained. htDNA-chip® can perform high-throughput next-generation sequencing to quickly learn the DNA sequence to quickly confirm the structure of the lead compound. htDNA-chip® significantly improves the screening efficiency of lead compounds.
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